Depends on what you mean.
If it is the same target variable just used in different sections of the code then yes else no.
The reason being that if you apply it to 2 or more different variables the algorithm will not be able to tell which one is causing the performance increase or decrease.
I have experimented with literally duplicating the algorithm (slightly changing the name of each variable in the algorithm) and assigning it’s output to a second variable.
And although this works to some extent it definitely reduces the accuracy and efficacy of the algorithm for the same reasons mentioned above.
